ABSTRACT
Objective: To investigate the genetic polymorphism of wild Anoectochilus roxburghii and its allied species by molecular marker and find out the genetic differences between them. Methods: Direct amplification of length polymorphism (DALP) was applied to evaluate the genetic variation of 18 populations of A. roxburghii and its allied species from different places. Results: Six pairs of primers were selected to detect 355 polymorphic loci in 18 populations, the percentage of polymorphic bands (PPB) was 100%, and the amplified average polymorphic locus by each pair of primers was 59.2. The percentage of PPB of 14 populations of A. roxburghii was 95.77%, its observed number of alleles (Na) was 1.2734, effective number of alleles (Ne) was 1.074, Nei's gene diversity (H) was 0.0562, Shannon's information index (I) was 0.0969, coefficient of gene differentiation (Gst) was 0.4230, and estimated gene flow (Nm) was 0.6819. Conclusion: The results indicate that A. roxburghii has larger genetic differentiation and lower gene flow among populations. Geographic isolation and wild resource loss may be the major causes of the limited gene flow.
ABSTRACT
Objective: To study the genetic diversity of Liriope muscari. Methods: The genetic diversity of 47 populations was analyzed by SRAP marker technique. Results: Fifteen primers amplified 323 polymorphic bands, and the average percentage of polymorphic bands reached to 88.47%. The average of polymorphism information content (PIC) was 0.90. Nei's gene diversity index (H) was 0.197 7 and Shannon's information index (I) was 0.319 0. Gene differentiation index (Gst) was 0.252 8. Cluster analysis using UPGMA method showed that genetic similarity coefficient ranged in 0.59-0.99. Conclusion: L. muscari shows higher genetic diversity and the majority of genetic variation occurs in populations.